Evaluation der Minimalen Alveolären Konzentration von Isofluran bei zwei transgenen Mausmodellen für die Alzheimer Krankheit

According to studies an estimated 5 per cent of people older than
65 years are suffering from Alzheimer’s disease. The pathology of
this disease might also influence some anaesthesiological
parameters. The aim of this study is to investigate the effect of
the Alzheimer- like pathology on the minimal alveolar concentration
(MAC) of isoflurane in two mice models of Alzheimer’s disease. The
MAC values of isoflurane were determined in twelve fifty month old,
transgenic APP23 male mice, which over-express a human amyloid
precursor protein with the Swedish double mutation and of twelve of
their non-transgenic littermates. In the same way, the MAC values
of twelve fifty month old, male transgenic APP51 mice, which carry
the same genetic construct as the APP23, however, without the
Swedish double mutation and of twelve of their non-transgenic
littermates were determined. The animals breathed isoflurane in
oxygen/air (FiO2=0,5) spontaneously through a nose chamber. The
motor reaction to toe-clamping was recorded at various
end-expiratory measured concentrations of isoflurane. An individual
MAC was defined as the average between the largest isoflurane
concentration permitting movement and the smallest concentration
preventing it. The average of this individual MAC values was taken
as the MAC of a test group. Statistical analysis was made with an
unpaired t-test (p