A chloride channel in rat and human axons

Current recordings from single chloride channels were obtained from
excised and cell-attached patches of rat and human axons. In rat
axons the channels showed an outwardly rectifying current-voltage
relationship with a slope conductance of 33 pS at negative membrane
potentials and 65 pS at positive potentials (symmetrical 150 mM
CsCl). They were measurably for cations (PNa/PCs/PCl=0.1/0.2/1).
Channel currents were independent of cytoplasmatic calcium
concentration. Inactivation was not observed and gating was weakly
voltage dependent. Cl− channels in human axons showed similar
gating behavior but had a lower conductance.