Determination of itraconazole and hydroxyitraconazole in plasma by use of liquid chromatography-tandem mass spectrometry with on-line solid-phase extraction

In this paper a method for the simultaneous quantification of the
anti-fungal drug itraconazole and its co-active metabolite
hydroxyitraconazole in plasma employing liquid chromatography
tandem-mass spectrometry and automated solid-phase extraction is
described. The method proved rugged, enables short turn-around
times and is highly specific. Since there is growing evidence for
the importance of therapeutic drug monitoring of itraconazole in
the prophylaxis and treatment of invasive fungal infections, the
method described here is of interest for a large number of tertiary
care hospital laboratories.