Comparative chromosome band mapping in primates byin situ suppression hybridization of band specific DNA microlibraries

A DNA-library established from microdissected bands 8q23 to 8q24.1
of normal human chromosomes 8 (Lüdecke et al., 1989) was used as a
probe for chromosomal in situ suppression (CISS-) hybridization to
metaphase chromosomes of man and primates including Hylobates lar
and Macaca fuscata. Comparative band mapping as first applied in
this study shows the specific visualization of a single
subchromosomal region in all three species and thus demonstrates
that synteny of the bulk sequences of a specific human chromosome
subregion has been conserved for more than 20 million years.