Specific metaphase and interphase detection of the breakpoint region in 8q24 of burkitt lymphoma cells by triple-color fluorescence in situ hybridization

Triple fluorescence in situ hybridization with a plasmid DNA
library from sorted human chromosomes 8 in combination with
bacteriophage clones flanking the breakpoint in 8q24 of the Burkitt
lymphoma cell line Jl was used for the specific delineation of this
breakpoint in individual tumor cells. With this approach,
tumor-specific breakpoints in translocation chromosomes can be
detected at all stages of the cell cycle with high specificity.