Chromosomal in situ suppression hybridization of immunologically classified mitotic cells in hematologic malignancies

Chromosomal in situ suppression (CISS) hybridization was performed
with library DNA from sorted human chromosomes 8, 9, 15, 17, 21,
and 22 on immunologically stained bone marrow cells of four
patients with a hematologic neoplasm, including two patients with
myelodysplastic syndrome and trisomy 8, one patient with
promyelocytic leukemia bearing the translocation
t(15;17)(q22;q11-12), and one patient with chronic myeloid leukemia
and the translocation t(9;22)(q34;q11). In all patients, the
results of conventional karyotype analysis could be confirmed by
one- or two-color CISS hybridization using the appropriate
chromosome-specific libraries. Our results show that CISS
hybridization can detect both numerical and structural chromosome
changes in immunologically classified cells with high specificity
and reliability. The fact that chromosome spreads of very poor
quality can now be included in such analyses is a decisive
advantage of this approach. In addition, the suitability of this
approach for interphase cytogenetics is discussed.