Molecular cloning, expression analysis and assignment of the porcine tumor necrosis factor superfamily member 10 gene (TNFSF10) to SSC13q34 -> q36 by fluorescence in situ hybridization and radiation hybrid mapping

We have cloned the complete coding region of the porcine TNFSF10
gene. The porcine TNFSF10 cDNA has an ORF of 870 nucleotides and
shares 85 % identity with human TNFSF10, and 75% and 72% identity
with rat and mouse Tnfsf10 coding sequences, respectively. The
deduced porcine TNFSF10 protein consists of 289 amino acids with
the calculated molecular mass of 33.5 kDa and a predicted pI of
8.15. The amino acid sequence similarities correspond to 86, 72 and
70% when compared with human, rat and mouse sequences,
respectively. Nor-them blot analysis detected TNFSF10-specific
transcripts (similar to 1.7 kb) in various organs of a 10-week-old
pig, suggesting ubiquitous expression. Real-time RT-PCR studies of
various organs from fetal (days 73 and 98) and postnatal stages
(two weeks, eight months) demonstrated developmental and
tissue-specific regulation of TNFSF10 mRNA abundance. The
chromosomal location of the porcine TNFSF10 gene was determined by
FISH of a specific BAC clone to metaphase chromosomes. This TNFSF10
BAC clone has been assigned to SSC13q34 -> q36. Additionally,
the localization of the TNFSF10 gene was verified by RH mapping on
the porcine IMpRH panel. Copyright (c) 2005S. KargerAG, Basel.